Study on the role of phosphate solubilizing fungi in. Lastly, the role of phosphate solubilizing microorganisms in aerobic rice cultivation is highlighted. Filamentous and phosphate solubilizing fungi relationships with some edaphic. Phosphate solubilizing bacteria psb function in soil phosphorus cycle, increasing the bioavailability of soil phosphorus for plants. A number of phosphate solubilizing bacteria and fungi act as plant growth promoters because of their ability to release iaa souchie et al. It is important to explore a highefficient phosphatesolubilizing approach with a kind of microorganisms. Phosphatesolubilizing microorganisms and their use springerlink. The fact that all identified psolubilizing fungi belong to the aspergillus or pene. The results revealed that phosphate solubilizing fungi a. Pdf phosphorus is an important nutrient required for plant growth, yield and adaptability to different ecological conditions.
After screening, 14 fungal isolates were obtained, which were further screened based. In vitro, potential of the psolubilizing activity by these psms were observed. Isolation and characterization of phosphofungi, and screening of. During the last two decades knowledge on phosphate solubilizing microorganisms increased significantly richardson 2001. Tofazzal islama,b, abhinandan deoraa, yasuyuki hashidokoa, atiqur rahmana, toshiaki itoa, and satoshi taharaa a graduate school of agriculture, hokkaido university, kitaku, sapporo 0608589, japan. In addition it can also solubilise k, zn, fe and mn and is also found to have antagonistic activity against select phytopathogens viz.
The biosolubilization of jordan phosphorite by the phosphatesolubilizing fungus aspergillus niger has been investigated. A number of bacterial and fungal isolates from soil were screened for phosphorus solubilization. The effect of the phosphate concentration in the liquid medium, the duration of biodecomposition, titratable acidity and the effect of preliminary mechanical activation on the process of dissolution have been studied. The analysis of phosphate solubilizing activity of the selected isolates were conducted qualitatively and quantitatively by plate screening method and broth culture method. Two psf njdl03 and njdl12 were isolated and identified as penicillium oxalicum and. Phosphate solubilizing bacteria psb have the potential to enhance phosphateinduced immobilization of metals to remediate contaminated soil. The effect of phosphate supply on phosphatase activity of. The present study was aimed at isolating and characterizing phosphate solubilizing fungi from different rhizospheres using both solid and liquid pikovskaya pvk medium. Please read the research publication on how phosphate solubilizing bacteria can reduce chemical inputs by at least 25%. Isolation and identification of potential phosphate. Phosphate solubilizing microorganisms principles and. In this study, two psf, talajx04 and aspnjx16, were isolated from the rhizosphere soil of moso bamboo phyllostachys edulis widely distributed in pdeficient areas in china and identified as talaromyces aurantiacus.
The integrated use of rock phosphate and psm add 2030 kg p2o5ha. Phosphate solubilizing bacteria psb play an important role in biogeochemical phosphorus cycling in both terrestrial and aquatic environments. Arbuscular mycorrhizal fungi amf and phosphate solubilizing pseudomonas bacteria psb could potentially interact synergistically because psb solubilize phosphate into a form that amf can absorb and transport to the plant. Phosphate solubilizing fungi play a noteworthy role in increasing the bioavailability of soil phosphates for plants. The present study focuses on the phosphatesolubilizing capacity of bacteria and fungi in rhizospheres soil samples obtained from. The selected strain was identified as aspergillus niger based on morphological and microscopic studies. The use of coldtolerant phosphate solubilizing microorganisms to enhance crop productivity in mountainous regions is examined, as are the ecological diversity and biotechnological implications of phosphate solubilizing microorganisms. Sep 05, 2016 phosphate solubilizing fungi play a noteworthy role in increasing the bioavailability of soil phosphates for plants. Phosphatesolubilizing bacteria psb function in soil phosphorus cycle, increasing the bioavailability of soil phosphorus for plants.
Isolation and characterization of phosphatesolubilizing. This reaction, appearing as a halo or clear zone on the plate, is used to assess the psolubilizing. Fig 1 growth parameter phosphate solubilizing fungi on some growth parameters,yield,p and n percentage in ground nut plants. Psb possess phosphate solubilizing ability and they can convert the insoluble phosphatic compounds. Phosphate solubilizing microbes have been reported for plant growth promoting and enhancing yield kapoor, mishra, and kuhkreja 1989.
Phosphate solubilizing bacteria and their role in plant. Phosphate solubilizing microorganisms naturally occurring rhizospheric phosphorus solubilizing microorganism psm dates back to 1903 khan et al. However, there is a limit on the amount of phosphate which can be added to the environment due to the issue of eutrophication. Phosphate solubilizing fungi psf have huge potentials in enhancing release of phosphorus from fertilizer. Biofertilization with mycorrhizal fungi and phosphate. Phosphate solubilizing activity is greater for fungi than bacteria. This study aimed to isolate a highefficient level of phosphatesolubilizing fungus from rhizosphere soil samples phosphate mines liuyang county, hunan province, china and apply it in solubilization of rps. Inoculation of phosphate solubilizing micro organisms increase crop yield by 510 %. Thus, an attempt was made to isolate and screen potential phosphate solubilizing bacteria psb which can able to solubilize phosphate and be used as biofertilzer in future. Mineral phosphate solubilization several reports have examined the ability of different bacterial species to solubilize insoluble inorganic phosphate compounds, such as tricalcium phosphate, dicalcium phosphate, hydroxyapatite, and rock phosphate 38. In soil phosphate solubilizing bacteria constitute 1 to 50%,while phosphate solubilizing fungi are 0.
This is due to either adsorption of soluble pi ions from the. Thus, the synthesis and discharge of organic acid by the psm strains into the surrounding. However, very little is known about the interactions between these two groups of microorganisms and how they influence the growth of each other. This study aimed to isolate a highefficient level of phosphate solubilizing fungus from rhizosphere soil samples phosphate mines liuyang county, hunan province, china and apply it in solubilization of rps. Biofertilization with mycorrhizal fungi and phosphate solubilizing microorganisms enhance effectiveness of phosphate fertilizers in tropical soils walter osorio and laura osorno abstract the low soil phosphate availability is one of the most serious constraints in tropical agriculture. Oct 31, 20 phosphorus is the second important key element after nitrogen as a mineral nutrient in terms of quantitative plant requirement. Phosphate solubilizing bacteria and their role in plant growth. Moreover, psolubilizing fungi do not lose the p dissolving activity upon repeated sub culturing under laboratory conditions as occurs with the psolubilizing bacteria sperber 1958a, b. Impact on growth and development of economically important plants. Isolation and characterization of two phosphatesolubilizing fungi. Evaluation of phosphate solubilizing fungi from mangrove.
Many soil microorganisms make p more available to plants by solubilizing. Evaluation of phosphate solubilizing fungi from mangrove soil. Nov 09, 2015 in soil phosphate solubilizing bacteria constitute 1 to 50%,while phosphate solubilizing fungi are 0. Phosphatesolubilizing bacteria employ different strategies to convert unavailable forms of phosphate into available forms. Isolation and characterization of a phosphatesolubilizing. This work was supported by grants from the national key. Microbial solubilization of fixed mineral phosphates in the soil is an important process in natural ecosystems and in agricultural soils. In this study, two psf, talajx04 and aspnjx16, were isolated from the rhizosphere soil of moso bamboo phyllostachys edulis widely distributed in pdeficient areas in china and identified as. Fungi isolation phosphate solubilizing fungi psf were isolated from 25 subsamples of the soils using selective media, pikovskaya medium pvk. The bacteria and fungi convert nonlabile p through either inorganic p solubilizing or organic p mineralization processes.
Phosphate solubilizing fungi, mangrove soils, biofertilizer, aspergillus niger, penicillium sp. The monovalent anion phosphate h 2 po 4is a major soluble form of inorganic phosphate, which usually occurs at lower ph. In the present work, seven most efficient phosphate solubilizing fungi were isolated from tea agroecosystem soil from barak valley, southern assam. This reaction, appearing as a halo or clear zone on the plate, is used to assess the p solubilizing. Isolation, screening and characterization of phosphate. Thus the application of p solubilizing fungi is recommended as a sustainable way for. Isolation and characterization of two phosphatesolubilizing. Solubilization of insoluble p by microorganisms was reported by pikovskaya. Evidence for naturally occurring phosphate solubilizing microorganism dates back to 1903 khan et al. This bioavailability of bacteria are used as biofertilizers since soil inorganic phosphorus in the 1950,s 26,28.
Phosphate solubilizing fungi isolated and characterized from teff. Collected rhizosphere soil samples were used for the isolation of phosphate solubilizing fungi on pikovskayas pkv agar medium, containing the following gl. Application of phosphate solubilizing fungi in the field has been reported to increase crop yield. Because of the negative charge of phosphate ions, they are quickly absorbed after weathering of clays or detritus particles, forming insoluble forms of aluminum. Isolation and application of salttolerant or halophilic psb will facilitate the development of salinealkali soilbased agriculture. A moderately halophilic bacterium was isolated from the sediment of daqiao saltern on the eastern coast of china, which also. Isolation of phosphate solubilizing bacteria and fungi. Effect of carbon and nitrogen source and concentration on. The study indicated that the bacteria have more solubilizing ability of. Phosphatesolubilizing fungi psf generally enhance available phosphorus p released from soil, which contributes to plants p requirement, especially in plimiting regions. It is important to explore a highefficient phosphate solubilizing approach with a kind of microorganisms. Pdf phosphate solubilizing fungi isolated and characterized from.
The focus is on understanding both the basic and applied aspects of phosphate solubilizing microorganisms and how phosphorusdeficient soils can be transformed into phosphorusrich ones by applying phosphate solubilizing microorganisms. Several strains of bacterial and fungal species have been described and investigated in detail for their. In vitro solubilization of inorganic phosphate by phosphate. The decrease in ph of the culture medium indicated the production of various organic acids by the culture. Phosphate solubilizing bacteria and fungi play an important role in p bioavailability and can be used to increase pue bolan et al. Phosphatesolubilizing bacteria and fungi in various cultivated and virgin alberta soils.
Isolation and identification of potential phosphate solubilizing bacteria from the rhizoplane of oryza sativa l. The native populations of phosphatesolubilizing bacteria and fungi were studied in. Isolation of phosphate solubilizing bacteria and fungi from. The phosphate solubilizing efficiency of isolated psb strain kupsb12 in pikovskayas broth indicated that the strain efficiently solubilized inorganic phosphate in the medium containing 0. Isolation of phosphatesolubilizing fungi from phosphate mines and their effect on wheat seedling growth article pdf available in applied biochemistry and biotechnology 1592. Some important cultural conditions were optimized, and optimal. Phosphate solubilizing fungi psf function in soil phosphorus cycle, increasing the bioavailability of soil phosphorus for plants. Solubilization, phosphorus, phosphorus solubilizing microorganisms. However as the ph of the soil environment increases the divalent and trivalent forms of pi hpo 42 and hpo 43 respectively occur. In general, phosphate solubilizing fungi produce more acids and consequently exhibit greater phosphatesolubilizing activity than bacteria in both liquid and solid media venkateswarlu et al. Phosphatesolubilizing fungi psf generally enhance available. A small number of studies investigated on the occurrence of phosphate solubilizing bacteria in the marine environment and fresh water environment like lake ecosystem. Soil microorganisms play an important role in making the phosphorus available to plants by mineralizing the organic phosphorus in the soil.
A moderately halophilic bacterium was isolated from the sediment of daqiao saltern on the eastern coast of china. In this regard, the ability of phosphofungi to dissolve insoluble phosphorus compounds is greater than that of phosphatesolubilizing bacteria. The native populations of phosphate solubilizing bacteria and fungi were studied in. Oscillospira traced through bergeys manual of determinative bacteriology. Qualitative screening of psm an efficient protocol was developed for qualitative screening of phosphate solubilizing bacteria, based upon visual observation. Number of microbial species plays key role in p solubilization these includes bacteria, fungi, actinomycetes and even algae. Among the whole microbial population in soil p, solubilizing bacteria comprise 150% and p solubilizing fungi 0. Soil micro organisms are rhizosphere varies considerably with responsible to intervene the soil plant species and nutritional status of soil processes such as decomposition, 23. Bacteria with phosphate solubilizing capacity alter. Phosphatesolubilizing microorganisms associated with the. Phosphate solubilization potential of rhizosphere fungi.
Oct 26, 2015 a number of bacterial and fungal isolates from soil were screened for phosphorus solubilization. Phosphate solubilizing bacteria an overview sciencedirect. Agnihotri vp 1970 solubilization of insoluble phosphates by some soil fungi. Introduction agriculture, occupies a unique position both conventional as. Although abundant in soils, in both organic and inorganic forms, its availability is restricted as it occurs mostly in insoluble forms. The identified fungi were tested for phosphate solubilization by the pikovskayas agar pvk selective media. A total of 59 fungi were originally obtained and were screened at the. Among the whole microbial population in soil, psb constitute 1 to 50 %, while phosphorus solubilizing fungi psf are only 0. The effecf of phosphate p and phytic acid s on phosphatase activity of phosphate solubilizing microorganisms in medium p supply pennicillium a. Phosphate is solubilized via organic acid synthesis and released by microorganisms puente et al.
All relevant data are within the paper and its supporting information files. The present study focuses on the phosphate solubilizing capacity of bacteria and fungi in rhizospheres soil samples obtained from. Qualitative screening of psm an efficient protocol was developed for qualitative screening of phosphatesolubilizing bacteria, based upon visual observation. Phosphate solubilizing biofertilizers as a component of inm many heterotropjic bacteria and fungi efficiently solubilize insoluble phosphate of soil and rock phosphates.
Effect of phosphate solubilizing fungi on growth and. Aspergillus niger and penicillium notatum were tested for their efficacy to solubilize tricalcium phosphate tcp in vitro as well as. Phosphate solubilizing bacteria can increase soil p availability. This study was aimed to isolate phosphate solubilizing bacteria from sugarcane waste compost. In most bacteria, the production of organic acids is shown to be related to the dissolution of mineral glucose to gluconic acid ga as the foremost mechanism for mineral phosphate solubilization in gramnegative bacteria. Effect of carbon and nitrogen source and concentration on rock phosphate dissolution induced by fungi laura osorno corresponding author. However, very little is known about the interactions between these two groups of microorganisms and how they influence.
Oct 31, 20 in soil, psolubilizing fungi constitute about 0. The acid and alkaline phosphatase activity were ranged from 8. Tofazzal islama,b, abhinandan deoraa, yasuyuki hashidokoa, atiqur rahmana, toshiaki itoa, and satoshi taharaa a graduate school of agriculture, hokkaido university, kitaku, sapporo 0608589, japan b present address. The study indicated that the bacteria have more solubilizing ability of inorganic insoluble phosphate than fungi. These fungal strains developed halozones on pvk agar, which is an indicator of phosphate solubilization. Inorganic phosphate pi is an essential nutrient for plant growth and development, however. Role of microorganisms in agriculture pdf the challenges of the coming decades.
Some important cultural conditions were optimized, and optimal conditions for temperature, glucose. One fungal strain was found to be efficient phosphorus solubilizer in pikovskaya medium. Sustainable agriculture involves the successful management of agricultural resources to. Pdf role of phosphate solubilizing fungi and microbes for.